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An aptamer-based electrochemical sensor that can distinguish influenza virus subtype H1 from H5
작성자 효소화학 연구실 작성일 2018-03-05 조회수 335
저자 : Jin-Moo Lee, Jun-Won Kim Ilhwan Ryu, Hye-Min Woo, Tae Gyun Lee, Woong Jung, Sanggyu Yim, and Yong-Joo Jeong 게제정보 : Adv. Aug. 2017 170778 , Research article

  • The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. Assessment by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide (ITO)-coated surface was specifically bound to the H1 subtype but not the H5 subtype, which was consistent with ELISA and FE-SEM. Further measurement of CV using various amount of H1 subtype provided detection limit of the immobilized aptamer, which showed that nanomolar scale of target protein was sufficient to produce the signal. These results indicated that the selected aptamer can be an effective probe for distinguishing the subtypes of influenza viruses by monitoring current changes



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