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  • 교수진

  • 정용주 교수
  • Professor
  • Chemistry of Enzyme
  • Ph.D. Seoul National University
  • Webpage :http://enzyme.kookmin.ac.kr/main.enzyme
  • E-mail : jeongyj@kookmin.ac.kr
  • Tel/ Room : (office) +82-2-910-5454 , (lab) +82-2-910-5098 , 5890
  • Location : (office) Room 610 [Dormitory D], (lab) Room 518 , 517 [Science B/D]

Education

B.S. in Department of Chemistry, College of Natural Science, Seoul National (1987-1993)


M.S. in Department of Chemistry, College of Natural Science, Seoul National (1993-1995)


Ph. D. in Department of Chemistry, College of Natural Science, Seoul National (1995-2000)

Career

Research Teaching Specialist III , Department of Biochemistry, University of Medicine and Dentistry of New Jersey (2000 ~ 2005)


Assistant Professor, Department of Bio and Nanochemistry, Kookmin University (2005 ~ 2009)


Associate Professor, Department of Bio and Nanochemistry, Kookmin University (2009 ~ present)

Research Topics

Biosensor Research


Drug Candidate Screening


Enzyme Mechanism


Selected publications

1. Joon Sig Choi, Eun Jung Lee, Young Hun Choi, Yong-Joo Jeong, and Jong Sang Park, “Poly(ethylene glycol)-block- poly(L-lysine) dendrimer : Novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA”, Bioconjugate Chem., 1999, 10, 62-65



2. Jin-Ho Choy, Seo-Young Kwak, Jong-Sang Park, Yong-Joo Jeong, and Josik-Portier, “Intercalative nanohybrids of nucleoside monophosphates and DNA in layered metal hydroxide”, J. Am. Chem. Soc. 1999, 121(6), 1399-1400.



3. Jin-Ho Choy, Jong-Sang Park, Seo-Young Kwak, Yong-Joo Jeong and Yang-Su Han, “Layered Double Hydroxide as Gene Reservoir”, Molecular Crystal and Liquid Crystal. 2000, 341, 425-429



4. Jin-Ho Choy, Seo-Young Kwak, Yong-Joo Jeong, and Jong-Sang Park, “Inorganic layered double hydroxides as non-viral vectors”, Angew. Chem. Int. Ed. 2000, 39(22), 4041-4045



5. Jin-Ho Choy, Seo-Young Kwak, Jong-Sang Park, and Yong-Joo Jeong, “Cellular uptake behavior of [g-32P] labeled ATP-LDH nanohybrids”, J. Mater. Chem., 2001, 11(6), 1671-1674



6. Seo-Young Kwak, Yong-Joo Jeong, Jong-Sang Park, and Jin-Ho Choy, “Bio-LDH nanohybrid for gene therapy”, Solid State Ionics, 2002, 151, 229-234



7. Yong-Joo Jeong, Dong-Eun Kim, and Smita S. Patel, “Kinetic Pathway of dTTP Hydrolysis by Hexameric T7 Helicase-primase in the Absence of DNA”, J. Biol. Chem., 2002, 277(46), 43778-43784



8. Yong-Joo Jeong, Dong-Eun Kim, and Smita S. Patel, “Nucleotide Binding Induces Conformational Changes in Escherichia coli Transcription Termination Factor Rho”, J. Biol. Chem., 2004, 279(18), 18370-18376



9. Yong-Joo Jeong, Mikhail K. Levin, and Smita S. Patel, “The DNA-Unwinding Mechanism of the Ring Helicase of Bacteriophage T7”, Proc. Natl. Acad. Sci. USA, 2004, 101(19), 7264-7269



10. Natalie M. Stano, Yong-Joo Jeong, Ilker Donmez, Padmaja Tummalapalli, Mikhail K. Levin and Smita S. Patel, “DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase”, Nature 2005, 435, 370-373



11. Jung-Chi Liao*, Yong-Joo Jeong*, Dong-Eun Kim, Smita S. Patel and George Oster, “Mechanochemistry of T7 DNA Helicase” J. Mol. Biol., 2005, 350(3), 452-475

* These authors contributed equally to this work.



12. Yong-Joo Jeong and Dong-Eun Kim, “Presteady State Kinetics of ATP Hydrolysis by Escherichia coli Rho Protein Monitors the Initiation Process” Bulletin of the Korean Chemical Society 2006, 27(2), 224-230



13. M. Asif-Ullah, Kyoung-Jae Choi, Kyung-Il Choi, Yong-Joo Jeong, and Yeon Gyu Yu, “Identification of compounds that inhibit the interaction between core and surface protein of hepatitis B virus” Antiviral Research 2006, 70(2), 85-90



14. Joshua L. Adelman*, Yong-Joo Jeong*, Jung-Chi Liao, Gayatri Patel, Dong-Eun Kim, George Oster, and Smita S. Patel, “Mechanochemistry of Transcription Termination Factor Rho” Molecular Cell 2006, 22, 611-621

* These authors contributed equally to this work.



15. Dong-Eun Kim and Yong-Joo Jeong, “Single-stranded DNA Enhances the Rate of Product Release During Nucleotide Hydrolysis Reaction by T7 DNA Helicase” Bulletin of the Korean Chemical Society 2006, 27(10), 1618-1622



16. Jong Wan Bae, Ju Hee Han, Mi So Park, Sun-Gu Lee, Eun Yeol Lee, Yong Joo Jeong, and Sunghoon Park, “Development of Recombinant Pseudomonas putida Containing Homologous Styrene Monooxygenase Genes for the Production of (S)-Styrene Oxide” Biotechnology and Bioprocess Engineering 2006. 11(6), 530-537



17. Ilker Donmez, Vaishnavi Rajagopal, Yong-Joo Jeong, and Smita S. Patel, “Nucleic Acid Unwinding by Hepatitis C Virus and Bacteriophage T7 Helicases Is Sensitive to Base Pair Stability” J. Biol. Chem. 2007, 282(29), 21116-21123



18. Kyoung Jin Jang, Yu-Jin Bae, Sung-Jong Jeon, Kyunghwa Kim, Jung-Hee Lee, Sung Su Yea, Sangtaek Oh, Yong-Joo Jeong, Dong-Eun Kim, “Nucleotide and Manganese Ion is Required for Chaperonin Function of the Hyperthermostable Group II Chaperonin α from Aeropyrum pernix K1” Bulletin of the Korean Chemical Society 2007, 28(12), 2261-2265



19. Kyoung Jin Jang, Na-Ra Lee, Woon-Seok Yeo, Yong-Joo Jeong*, and Dong-Eun Kim*, “Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase” Biochemical and Biophysical Research Communications 2008, 366(3), 738-744

* Corresponding authors.



20. Jong Wan Bae, Seunghee Shin, S. Mohan Raj, Song Eun Lee, Sun-Gu Lee, Yong-Joo Jeong, and Sunghoon Park, “Construction and Characterization of a Recombinant Whole-cell Biocatalyst of Escherichia coli Expressing Styrene Monooxygenase under the Control of Arabinose Promoter” Biotechnology and Bioprocess Engineering 2008. 13(1), 69-76



21. Ivan Rasnik, Yong-Joo Jeong, Sean A McKinney, Vaishnavi Rajagopal, Smita S Patel, and Taekjip Ha, “Branch migration enzyme as a Brownian ratchet” EMBO Journal 2008, 27, 1727–1735



22. So-Yeon Kim, Niraikulam Ayyadurai, Mi-Ae Heo, Sunghoon Park, Yong Joo Jeong, Sun-Gu Lee, “Improving the Productivity of Recombinant Protein in Escherichia coli Under Thermal Stress by Coexpressing GroELS Chaperone System” J. Microbiol. Biotechnol. 2009, 19(1), 72-77



23. Chaewoon Lee, Jin Moo Lee, Na-Ra Lee, Bong-Suk Jin, Kyoung Jin Jang, Dong-Eun Kim, Yong-Joo Jeong*, and Youhoon Chong*, “Aryl diketoacids (ADK) selectively inhibit duplex DNA-unwinding activity of SARS coronavirus NTPase/helicase” Bioorganic & Medicinal Chemistry Letters 2009, 19(6), 1636-1638

* Corresponding authors.



24. Yun-Ji Yeo, Seunghee Shin, Sun-Gu Lee, Sunghoon Park, Yong-Joo Jeong, “Production, Purification, and Characterization of Soluble NADH-Flavin Oxidoreductasae (StyB) from Pseudomonas putida SN1” J. Microbiol. Biotechnol. 2009, 19(4), 362-367



25. Jong Wan Bae, Miso Park, Yong Joo Jeong, Sunghoon Park and Sun-Gu Lee, “Colorimetric monitoring of the activity of recombinant Escherichia coli expressing styrene monooxygenase” Journal of Industrial and Engineering Chemistry 2009, 15(4), 520-523



26. Chaewoon Lee, Jin Moo Lee, Na-Ra Lee, Dong-Eun Kim, Yong-Joo Jeong*, and Youhoon Chong*, “Investigation of the pharmacophore space of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) NTPase/helicase by dihydroxychromone derivatives” Bioorganic & Medicinal Chemistry Letters 2009, 19(16), 4538-4541

* Corresponding authors.



27. Na Ra Lee, A Ram Lee, Bokhui Lee, Dong Eun Kim*, and Yong Joo Jeong*, “ATP Hydrolysis Analysis of Severe Acute Respiratory Syndrome (SARS) Coronavirus Helicase” Bulletin of the Korean Chemical Society 2009, 30(8), 1724-1728

* Corresponding authors.



28. Yong-Joo Jeong, Kkothanahreum Park, and Dong-Eun Kim, “Isothermal DNA amplification in vitro: the helicase-dependent amplification system” Cellular and Molecular Life Sciences 2009, 66(20), 3325-36



29. Jong-Wan Bae, Eun-Hee Doo, Seung-Hee Shin, Sun-Gu Lee, Yong-Joo Jeong, Jin-Byung Park, Sunghoon Park, “Development of a recombinant Escherichia coli-based biocatalyst to enable high styrene epoxidation activity with high product yield on energy source” Process Biochemistry 2010, 45(2), 147-152



30. Il-Hyun Kim, Seonmi Shin, Yong-Joo Jeong, Sang Soo Hah, “Two-step synthesis of 5′-deoxy-5′-thioguanosine-5′-monophosphorothioate and its incorporation efficiency into 5′-terminus of RNA for preparation of thiol-functionalized RNA” Tetrahedron Letters, 2010, 51( 26), 3446-3448



31. Na-Ra Lee, Hyun-Mi Kwon, Kkothanahreum Park, Sangtaek Oh, Yong-Joo Jeong* and Dong-Eun Kim*, “Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13” Nucleic Acids Research, 2010, 38 (21), 7626-7636

* Corresponding authors.



32. S. K. Bae, Y.-J. Jeong, C. Lee, K.-H. Liu, “Identification of human UGT isoforms responsible for glucuronidation of efavirenz and its three hydroxy metabolites” Xenobiotica, 2011, 41(6), 437-444



33. Mi Kyoung Kim, Mi-Sun Yu, Hye Ri Park, Kyung Bo Kim, Chaewoon Lee, Suh Young Cho, Jihoon Kang, Hyunjun Yoon, Dong-Eun Kim, Hyunah Choo, Yong-Joo Jeong*, Youhoon Chong*, “2,6-Bis-arylmethyloxy-5-hydroxychromones with antiviral activity against both hepatitis C virus (HCV) and SARS-associated coronavirus (SCV)” European Journal of Medicinal Chemistry, 2011, 46(11), 5698-5704

* Corresponding authors.



34. Seong-Je Cho, Hye-Min Woo, Ki-Sun Kim, Jong-Won Oh, Yong-Joo Jeong, “Novel system for detecting SARS coronavirus nucleocapsid protein using an ssDNA aptamer” Journal of Bioscience and Bioengineering, 2011, 112(6), 535-540



35. Mi-Sun Yu, June Lee, Jin Moo Lee, Younggyu Kim, Young-Won Chin, Jun-Goo Jee, Young-Sam Keum, Yong-Joo Jeong, “Identification of myricetin and scutellarein as novel chemical inhibitors of the SARS coronavirus helicase, nsP13” Bioorganic & Medicinal Chemistry Letters 2012, 22(12), 4049-4054



36. Young-Sam Keum, Yong-Joo Jeong, “Development of chemical inhibitors of the SARS coronavirus: Viral helicase as a potential target” Biochemical Pharmacology 2012, 84(10), 1351-1358

Presentations

“Single stranded DNA aptamer Specifically Binding to Influenza non structural 1 (NS1) protein” (poster presentation), The Commemorative International Cobference for the 20th Anniversary of Korean Society of Life Science, Busan, Korea, Oct. 27 – 28, 2011



“Avian Influenza A virus NS1 (H5N1) protein escape from RIG-I mediate host innate antiviral responses” (poster presentation), The Commemorative International Cobference for the 20th Anniversary of Korean Society of Life Science, Busan, Korea, Oct. 27 – 28, 2011



“Development of ssDNA Aptamers that can distinguish between H1N1 and H5N1” (poster presentation), The Commemorative International Conference for the 20th Anniversary of Korean Society of Life Science, Busan, Korea, Oct. 27 – 28, 2011



“Influenza A virus NS1 protein to evade recognition by the host viral RNA sensor RIG-I” (poster presentation), The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, Oct. 6 – 7, 2011



“Isolation of ssDNA aptamer against Influenza Virus Non structure 1 protein” (poster presentation), The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, Oct. 6 – 7, 2011



“Development of ssDNA Aptamer that make a distinction between Hemagglutinin subtype H1 and H5” (poster presentation), The 23rd Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, Oct. 6 – 7, 2011



“Single-molecule study for cooperative translocation motion of nsP13 helicase during duplex DNA unwinding” (poster presentation), The 108th National Meeting of the Korean Chemical Society, Daejeon, Korea, Sep. 28 – 30, 2011



“ssDNA Aptamer that can distinguish between H1N1 and H5N1 by recognize Hemagglutinin subunit HA1” (poster presentation), The 108th National Meeting of the Korean Chemical Society, Daejeon, Korea, Sep. 28 – 30, 2011



“In vitro selection of DNA aptamers against influenza non structural 1 (NS1) protein” (poster presentation), The 108th National Meeting of the Korean Chemical Society, Daejeon, Korea, Sep. 28 – 30, 2011



“In vitro selection of DNA aptamers against influenza non structural 1 (NS1) protein” 2011 KSBMB annual meeting, Seoul, Korea, May 16 – 18, 2011



“Isolation of ssDNA aptamers against spike protein of SARS coronavirus” 2011 KSBMB annual meeting, Seoul, Korea, May 16 – 18, 2011



“Discovery of chemical compounds that inhibit SARS-CoV helicase activity with high-throughput screening” 2011 KSBMB annual meeting, Seoul, Korea, May 16 – 18, 2011



“Quantitative isotopic nitrocellulose filter binding assays: SARS CoV helicase-nucleic acid interactions” (poster presentation), The 106th National Meeting of the Korean Chemical Society, Daegu, Korea, Oct. 14 – 15, 2010



“The studies of interaction between each domain of human RIG-I and AIV NS1” (poster presentation), The 106th National Meeting of the Korean Chemical Society, Daegu, Korea, Oct. 14 – 15, 2010



“Interaction of RIG-I with AIV NS1 in the presence of dsRNA” (poster presentation), The 22nd Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, Oct. 7 – 8, 2010



“Isotopic filter binding assay for SARS Coronavirus helicase-nucleic acid complexes” (poster presentation), The 22nd Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, Oct. 7 – 8, 2010



“The effects of substrate composition on helicase unwinding activity of SARS coronavirus nsP13” (poster presentation), The 105th National Meeting of the Korean Chemical Society, Incheon, Korea, Apr. 29 – 30, 2010



“In Vitro Selection of DNA Aptamers against Nucleocapsid protein of SARS Coronavirus” (poster presentation), The 105th National Meeting of the Korean Chemical Society, Incheon, Korea, Apr. 29 – 30, 2010



“Dihydroxychromone derivatives selectively inhibit duplex DNA-unwinding activity of Severe Acute Respiratory Syndrome Coronavirus NTPase/Helicase” (poster presentation), The 104th National Meeting of the Korean Chemical Society, Daejeon, Korea, Oct. 28 – 30, 2009



“Characterization and isolation of RNA aptamer against Avian Influenza NS1 protein” (poster presentation), The 104th National Meeting of the Korean Chemical Society, Daejeon, Korea, Oct. 28 – 30, 2009



“Kinetic measurement of nucleic acid unwinding by Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) helicase” (poster presentation), The 104th National Meeting of the Korean Chemical Society, Daejeon, Korea, Oct. 28 – 30, 2009



“Studies of interaction between RIG-I and NS1 in the presence and absence of nucleic acids” (poster presentation), The 104th National Meeting of the Korean Chemical Society, Daejeon, Korea, Oct. 28 – 30, 2009



“Biochemical studies and inhibitor screening of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) helicase” (oral presentation), Asia-Korean Conference on Advanced Science & Technology 2009, Yanji, China, Aug. 28 – 29, 2009



“Biochemical analysis of ATP hydrolysis and DNA unwinding activities of the Severe Acute Respiratory Syndrome Coronavirus (SARS CoV) helicase” The 66th KSBMB annual meeting 2009, Seoul, Korea, May 12 – 13, 2009



“SARS coronavirus helicase is specifically inhibited by Aryl diketoacids analogues” The 66th KSBMB annual meeting 2009, Seoul, Korea, May 12 – 13, 2009



“Characterization of severe acute respiratory syndrome (SARS) coronavirus helicase” (poster presentation), The 103rd National Meeting of the Korean Chemical Society, Seoul, Korea, Apr. 16 – 17, 2009



“High-throughtput screening assay of Severe Acute Respiratory Syndrome Coronavirus Helicase for finding inhibitors” (poster presentation), The 103rd National Meeting of the Korean Chemical Society, Seoul, Korea, Apr. 16 – 17, 2009



“ATP hydrolysis assays of SARS coronavirus helicase in the presence of single-stranded nucleic acids” (poster presentation), The 102nd National Meeting of the Korean Chemical Society, Jeju, Korea, Oct. 16 – 17, 2008



“Enzymatic cross-linking of StyA and StyB by Microbial Transglutaminase” (poster presentation), The 101st National Meeting of the Korean Chemical Society, Ilsan, Korea, Apr. 17 – 18, 2008



“The study of steady state ATP hydrolysis by SARS Coronavirus helicase” (poster presentation), The 101st National Meeting of the Korean Chemical Society, Ilsan, Korea, Apr. 17 – 18, 2008



“Inhibition of Severe acute respiratory syndrome (SARS) coronavirus helicase with RNA aptamers containing conserved sequence” (poster presentation), The 101st National Meeting of the Korean Chemical Society, Ilsan, Korea, Apr. 17 – 18, 2008



“Enzymatic properties of Severe Acute Respiratory Syndrome (SARS) Coronavirus helicase” (poster presentation), The 100th National Meeting of the Korean Chemical Society, Daegu, Korea, Oct. 18 – 19, 2007



“Enzymatic cross-linking of two-component monooxygenase encoded by StyA and StyB” (poster presentation), The 100th National Meeting of the Korean Chemical Society, Daegu, Korea, Oct. 18 – 19, 2007



“In vitro selection of RNA aptamer that is specific for Severe Acute Respiratory Syndrome (SARS) Coronavirus helicase” (poster presentation), The 100th National Meeting of the Korean Chemical Society, Daegu, Korea, Oct. 18 – 19, 2007



“T7 helicase recovers dsDNA unwinding ability by DNA synthesis” (oral presentation), 2006 International Meeting of the Federation of Korean Microbiological Societies, Seoul, Korea, Oct. 19 – 20, 2006



“Translocation of Bacteriophage T7 Helicase-Primase along Duplex DNA” (poster presentation), Biophysical society 48th annual meeting at Baltimore, Maryland, Feb. 14 - 18, 2004



“Mechanism of DNA unwinding by bacteriophage T7 helicase-primase : Investigation of the role of the 3’-tail, rate of unwinding and step-size” (poster presentation), FASEB summer research conference at Saxton River, Vermont, Jun. 28 – Jul. 3, 2003



“Kinetics of dsDNA unwinding and dTTP hydrolysis of T7 helicase-primase” (platform presentation), Biophysical society 47th annual meeting at San Antonio, Texas, Mar. 1 - 5, 2003



“Kinetic mechanism of nucleotide binding and hydrolysis by pre-assembled T7 bacteriophage helicase” (poster presentation), Department of Biochemistry Retreat at New Brunswick, NJ, Sep. 13, 2002



“Kinetic mechanism of nucleotide binding and hydrolysis by T7 bacteriophage gp4A'” (poster presentation), FASEB summer research conference at Saxton River, Vermont, Jul. 7 - 12, 2001



“Kinetic analysis of dsDNA unwinding and nucleotide hydrolysis by T7 bacteriophage helicase gp4” (poster presentation), Department of Biochemistry Retreat at Warren, NJ, Oct. 27, 2000



“Partial Purification and Characterization of Glucose-6-Phosphatase from Rat Liver Microsome” (poster presentation), Annual Conference of Korean Society of Molecular Biology, Korea Academic Institute of Science and Technology, Taejeon, Korea, 1993

Honors and Awards

- Selected for inclusion in 2009 and 2011 editions of Marquis Who’s Who in the World.

Memberships

- Korean Chemical Society

- Korean Society for Biochemistry & Molecular Biology

- Korean Society for Molecular and Cellular Biology